Journal: Bioorganic & medicinal chemistry
Article Title: Selective photodepletion of malignant T cells in extracorporeal photopheresis with selenorhodamine photosensitizers
doi: 10.1016/j.bmc.2016.05.071
Figure Lengend Snippet: Non-stimulated PBMCs were combined 5:1 with HUT-78 cells. Samples were then photodepleted (PD) with 7.5 × 10−8 M selenorhodamine 5–10 and 5 J cm−2 light. Cell survival was measured 18 h after light exposure and compared to control (non-PD samples) by FACS analysis. The bar graphs represent percent survival of malignant HUT-78, the CD3+ population, and the CD4+ and CD8+ subsets of CD3+ cells after photodepletion with selenorhodamines (a) 5 and 6 and (b) 7–10. The bars represent the mean of three replicate experiments. Error bars are the SEM.
Article Snippet: The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); and α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96).
Techniques: